作　　者： ; ; ; ;

机构地区： 广东海洋大学农学院

出　　处： 《Plant Diseases and Pests》 2010年第2期5-9,共5页

摘　　要： [目的]为了解RS261菌株诱导辣椒抗疫病的生理生化机理。[方法]测定用RS261菌株处理后辣椒果内可溶性蛋白、丙二醛(MDA)含量及过氧化氢酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)活性的变化。[结果]RS261菌株对辣椒苗可溶性蛋白含量和防御性酶活性无不良影响。疫霉和菌液+疫霉2个处理的辣椒苗MDA含量及SOD、CAT和POD活性在处理后呈不断上升趋势,只是菌液+疫霉处理的上升量比疫霉处理的低,而可溶性蛋白含量和PAL活性变化呈现不同趋势,菌液+疫霉处理的辣椒苗内可溶性蛋白含量和PAL活性随时间推移不断上升,而仅接病菌处理却不断下降。[结论]RS261菌株可以在一定程度上消除病原菌对辣椒体内活性氧清除相关酶(SOD、CAT和POD)的酶活性,共同接种后可诱导寄主体内PAL活性的变化,从而提高寄主的抗性。 [ Objective] The research aimed at understanding the physiological and biochemical mechanism of resistance against phytophthora blight of pepper by strain RS261. [Method] The content of soluble prctein,MDA and activity of SOD, CAT, POD and PAL in pepper fruit treated with RS261 strain were tested in three treatments （Treatment 1 ： inoculation of RS261. Treatment 2： inoculation of P. capsici. Treatment 3： inoculation of both P. capsici and RS261 ）. [Result] Treatment 1 had no harm to the content of soluble protein and the activities of defensive enzyme. MDA content and POD, SOD, CAT activity in Treatment I and 2 continuously increased, and the increasing rate of Treatment I was more higher. Changes of soluble protein content and PAL activity of treated pepper fruit were different after different treatments,the content of soluble protein content and PAL activity in pepper fruit in Treatment 3 increased continuously with time while that in Treatment 3 reduced. [ Conclusion] RS261 strain could eliminate the effect of pathogen on the activities of active oxygen scavenging enzymes （ SOD, CAT and POD）, which could induce changes of PAL activity in host, and enhance host-plant resistance.

关 键 词： 菌株 丙二醛 过氧化物酶 过氧化氢酶 苯丙氨酸解氨酶

领　　域： [农业科学]